027 Profilin-1 prevents psoriasis pathogenesis through IκBζ regulation

Profilin-1 (PFN-1) is an actin-binding protein that regulates actin polymerization, cell proliferation, apoptosis, angiogenesis, and carcinogenesis. Its dysregulation has been reported in diverse pathologic diseases; however, the role of PFN-1 psoriasis not yet elucidated. In this study, we demonstrate expression increased both skin serum patients with psoriasis. was markedly expressed epidermis psoriatic lesions its positively correlated severity. The human keratinocytes induced by IL-17A TNF-α stimulation, but only presence secreted out cell. addition, knockdown shRNA resulted altered psoriasis-associated inflammatory markers, HBD-2, S100A7, S100A9, Ki67, recombinant suppressed IL-17A-induced response keratinocytes. Interestingly, also IκBζ, important player immune Considering these facts, it suggested balance intracellularly or extracellularly for IκBζ regulation pathogenesis Our results showed acts as a negative regulator inflammation through suppression IκBζ. Furthermore, epidermal hyperplasia observed psoriasis-like reconstructed equivalents were reduced PFN-1, which indicates aberrant development due to response. conclusion, our findings can be used biomarker disease severity, allude considered possible target suppressing preventing onset alleviating symptoms.